Responsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferase
نویسندگان
چکیده
منابع مشابه
Poliovirus replicase stimulation by terminal uridylyl transferase.
In an in vitro poliovirus replication system, purified viral polymerase, plus sense virion RNA, and a host factor have been previously shown to be necessary for the transcription of minus strands. We have found that a partially purified eukaryotic initiation factor-2 (eIF-2) fraction from rabbit reticulocytes can replace HeLa host factor in the replicase reaction. This enzyme preparation contai...
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Synthetic oligonucleotides prepared from known DNA or amino acid sequences can be used as gene probes for detection of DNA and RNA. Short oligonucleotide probes can discriminate between two sequences which differ by only a single nucleotide (1). Biotin is the most frequently used non-radioactive reporter group for labelling of nucleic acids. Enzymatic and/or chemical synthesis have been used to...
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Terminal uridylyl transferases (TUTs) are responsible for the post-transcriptional addition of uridyl residues to RNA 3' ends, leading in some cases to altered stability. The Schizosaccharomyces pombe TUT Cid1 is a model enzyme that has been characterized structurally at moderate resolution and provides insights into the larger and more complex mammalian TUTs, ZCCHC6 and ZCCHC11. Here, we repor...
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ژورنال
عنوان ژورنال: Chemical Communications
سال: 2020
ISSN: 1359-7345,1364-548X
DOI: 10.1039/d0cc05092j